![]() Upon completion of the course, students have extensive skills with sequence analysis tools and are prepared for their own laboratory projects or bioinformatics software creation. Addgenes plasmid information pages recommend 5’ and 3’ sequencing primers. Throughout the semester, interesting biological questions are addressed by analyzing sequences, searching databases, using sophisticated software, and interpreting results. The free text search will scan for complete and partial matches to gene names, gene synonyms, gene descriptions, external (UniProt, Ensembl, NCBI Entrez Gene). Sequence verification of important plasmid features (such as the gene/insert, fusion proteins, point mutations, deletions, etc.) involves selecting one or more unique oligonucleotide primers that flank the regions of your plasmid that you wish to confirm. Students learn how to evaluate data sources and choose the correct paths to solutions. There are several steps in analyzing a protein. Classes consist of lecture and extensive hands-on work using mainstream web-based bioinformatics tools. The majority of protein sequence analysis today uses mass spectrometry. This course teaches the bioinformatics skills used in academic, biotech, and pharmaceutical laboratories for analyzing individual DNA and protein sequences. The details of this data reveal basic information such as gene and protein structures, and may lead us to major discoveries like gene-disease associations. This work exposes the collective nature of physical constraints on protein function and clarifies its link with sequence analysis, enabling a general practical. ![]() With breakthroughs in biotechnology such as high-throughput and inexpensive DNA sequencing, we are collecting vast amounts of data that will be analyzed for years to come. Protein Structure Analysis - Preparation and Characterization is a compilation of practical approaches to the structural analysis of proteins and peptides. ![]()
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